Preparation of Novel Shell Cross-linked Thermoresponsive Hybrid Micelles with Anti-tumor Efficacy
نویسندگان
چکیده
The shell cross-linked (SCL) thermoresponsive hybrid poly(N-isopropylacrylamide-co-aminoethyl methacrylate)b-polymethyl methacrylate (P(NIPAAm-co-AMA)-bPMMA) micelle consisting of a cross-linked thermoresponsive hybrid shell and a hydrophobic core domain was fabricated via a two-step process: micellisation of P(NIPAAm-co-AMA)-b-PMMA in aqueous solution followed by cross-linking of the hydrophilic shell layer using a novel inorganic cross-linker. The SCL micelle showed reversible dispersion/aggregation in response to the temperature cycles through the lower critical solution temperature (LCST) of the thermoresponsive hybrid shell at 36 C, observed by turbidity measurements. The inorganic compound used as a cross-linker has anti-tumor efficacy; and the resultant SCL micelle exhibited a remarkable cytotoxic effect against HeLa cells with a very low IC50.
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